Using the 2D-electrophoresis approach we identified proteins from spots with a relatively highly significant adjusted p-value as a result of extended fasting: 5 spots in plasma, 2 spots in PBMC, 1 spot in saliva and 2 spots in urine (see Table 2 and Figure 2 ).
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2D-electrophoresis and multiplex immunoassay proteomic analysis of different body fluids and cellular components reveal known and novel markers for extended fasting.
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