Gill transcriptome response to changes in environmental calcium in the green spotted puffer fish.

Furthermore, there was a highly significant positive correlation (r = 0.932, p = 3.7 × 10 -11 ) for the relative expression of the 8 genes relative to control (Figure 3 ) between qPCR and SuperSAGE, indicating an overall concordance between the two techniques in detecting differential expression.

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