highly significantP <0.001
In a mixed effect model including time step 1 and time step 4 in one model (see statistical analyses), the interaction term between insecticide treatment×time step was highly significant ( F 1,13 = 20.98, P <0.001), providing evidence that the effect of insecticide treatment was not constant across the sampling dates, with lower aphid abundances in the treated fields at the first survey (first time step), but higher aphid abundances in the treated fields at the last survey.