Role of the single-stranded DNA-binding protein SsbB in pneumococcal transformation: maintenance of a reservoir for genetic plasticity.

if each cell could internalize up to half a genome equivalent of ssDNA, then the probability of repair of chromosome damages using internalized ssDNA as template [32] would become highly significant.

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Whatever the concentration of plasmid DNA, ssbB Δ 7 and wildtype cells behaved similarly; a possible trend toward a ∼2-fold higher transformation rate in ssbB Δ 7 than in wildtype cells at high plasmid DNA concentrations was tentatively attributed to the increased protection of ssDNA in the former cells ( Figure 5 ).

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