One is enough: in vivo effective population size is dose-dependent for a plant RNA virus.

PLoS Pathog · 2011 · PMC3131263 · PMID 21750676

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an overall trendno p-value reported

Treating each estimate of κ as a replicate, we can test whether an overall trend exist to deviate from the IAH expectation.

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Quoted from the open-access full text in Europe PMC under the licence the publisher applied. The sentence is reproduced exactly as published; the emphasis is ours.