The third protein (PTH2) was found to be largely specific for Actinobacteria (A) and only 4 hits for Archaea (R) and 1 hit for Bacillus (B), which are barely significant, were observed.
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Origin of diderm (Gram-negative) bacteria: antibiotic selection pressure rather than endosymbiosis likely led to the evolution of bacterial cells with two membranes.
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