highly significantp<0.001
This was coupled to a significantly reduced ability to silence the wild-type target leading to highly significant 2.23- (p<0.001), 1.71- (p = 0.02), 1.96- (p = 0.01), 1.81- (p = 0.009) and 1.83-fold (p = 0.01) discrimination for constructs p11_9C, p11_9U, p11_10U, p11_10A, p11_10G respectively.