Tilt-pair analysis of images from a range of different specimens in single-particle electron cryomicroscopy.

The images and 3D map are from Lau and Rubinstein, in which the plot of the average residual on the tilted particles showed a highly significant phase difference of 14.9° between the structure with the correct hand and one with the opposite hand. 31 As expected, the individual TPPP shows that most of the particle image pairs have reasonably well-determined orientations: 27 particle pairs (54%) have orientations correct to within 16°, with 72% within 40° and with only 20% having a substantial out-of-plane error in one of the images in the pair.

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