Reduced mRNA secondary-structure stability near the start codon indicates functional genes in prokaryotes.

Genome Biol Evol · 2012 · PMC3269970 · PMID 22138151

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highly significantP = 3.6×10 − 9actually significant

The mean Z Δ G was 0.28 for genes with low CAI and 0.45 for genes with high CAI; the difference between these two groups was highly significant (two-sample t test, t = − 5.91, df = 4,159, P = 3.6×10 − 9 ).

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