Diversity measures in environmental sequences are highly dependent on alignment quality--data from ITS and new LSU primers targeting basidiomycetes.

PLoS One · 2012 · PMC3283731 · PMID 22363808

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highly significantp = 0.00not a probability at all

Highly significant differences (p = 0.00) between the index of substitution saturation (Iss) and the critical Iss (Iss.c) were observed for all alignments and site types other than ITS when examining all sites and treating gaps as unknown nucleotides (in which case p = 0.54).

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