Barely Significant
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"Cost" of virginity in wild Drosophila melanogaster females.

Ecol Evol · 2011 · PMC3287337 · PMID 22393526

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highly significantno p-value reported
By both tests, Log-Rank (χ 2 = 15.16, df = 1, P < 0.0001) and the Wilcoxon (χ 2 = 12.22, df = 1, P < 0.0005), the difference in survival in the first replication was highly significant, with mated females living longer.

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