Declining extra-pair paternity with laying order associated with initial incubation behavior, but independent of final clutch size in the blue tit.

Moreover, the interaction effect between laying order and experimental treatment on hatchability among the first ten eggs was almost significant (coefficient ± SE = 0.19 ± 0.10, χ 2 = 3.70, df = 1, P = 0.054), suggesting that early laid eggs in the experimental group, which were stored the longest, were least likely to hatch.

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Incubation and the number of eggs laid Our manipulation had a highly significant effect on the total number of eggs laid, as the experimental females laid an average of 2.1 more eggs than the control females (mean ± SE; control: 12.4 ± 0.19, n = 40; experimental: 14.5 ± 0.27, n = 42; t = −6.20, df = 80, P < 0.001).

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