Enumeration of Mycobacterium avium subsp. paratuberculosis by quantitative real-time PCR, culture on solid media and optical densitometry.

The absolute numbers of MAP determined by F57qPCR were approximately two log 10 greater than the CFU counts from culture at respective ODs (Figure 1 ) and a highly significant statistical difference ( P < 0.0001) between all the compared samples was observed.

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