Improved detection of bifidobacteria with optimised 16S rRNA-gene based pyrosequencing.

regular universal primers) there was a highly significant difference between the read abundance of bifidobacteria using ‘bifidobacteria-optimised’ primers compared to regular primers ( P = 0.039, t = 0.0026, with Bonferonni correction for multiple testing), but no significant differences between any of the other OTUs ( P >1.4).

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