When structure was run with populations from cluster I only, three highly significant clusters (K = 3) were identified ( Figure 1b ) corresponding to samples from the following areas: BR-SW: southwest Brazil (Uruguaiana, Alegrete and Itaqui); MED: Mediterranean localities [southern Spain (Granada and Malaga), Greece and Tunisia] and AF+EU: eastern Africa (AF) plus European localities (EU) apart from those included in MED ( Table 1 ).
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Test of colonisation scenarios reveals complex invasion history of the red tomato spider mite Tetranychus evansi.
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