High-anxious individuals show increased chronic stress burden, decreased protective immunity, and increased cancer progression in a mouse model of squamous cell carcinoma.

Importantly, for all comparisons where one of the two analyses, t -test or regression slope, showed a statistical trend, the remaining analysis showed statistical significance, the only exceptions being gene expression in UV exposed skin at week 17 for IFN-γ and at week 31 for the ratios of CTACK/CCL22 and (IL-12+IFN-γ)/(IL-4+IL-10), for which the t -test was not significant and the regression slopes only showed statistical trends.

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The difference between mice showing the high- versus low- anxious behavioral phenotype on each test was highly significant (p < = 0.0001) only when the above- versus below- median groups were compared for the test on which the median split was based, indicating independence among the behavioral measures.

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