Fluorescence intensity measurements specifically in this perinuclear region compared to the plasma membrane revealed a highly significant approximately two-fold increase in perinuclear GFP expression when Rheb is overexpressed with CG1139-GFP ( Figures 8E and 8F ).
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Proton-assisted amino acid transporter PAT1 complexes with Rag GTPases and activates TORC1 on late endosomal and lysosomal membranes.
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