highly significantP = 0.01
Genomic qPCR analysis of the g20 population samples using probes against MAD1 and MAD2 gene sequences found that: (i) strains with different MAD2 : MAD1 ratios were not uniformly distributed across the stable and unstable strains (P = 0.027, Figure 5C ); and (ii) 18 aneuploid strains with a MAD2 : MAD1 ratio of 0.5 all fell into the ‘ploidy unstable’ category, whereas all 11 ‘ploidy stable’ aneuploid strains had a MAD2 : MAD1 ratio of 1 or higher, indicating a highly significant association of a MAD2 : MAD1 ratio of 0.5 with CIN (P = 0.01, Figure 5D ).