Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis.

The profiling results were subjected to a supervised clustering analysis, and the 13 lectins that showed highly significant differential binding between pluripotent and non-pluripotent cell types ( P < 0.05, t -test) were identified for a “priority lectin” list ( Supplementary information, Table S1 ).

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We observed an interesting trend in the non-pluripotent cells: cells of similar lineages tended to be clustered more closely together in their hydrophobic protein glycan profiles than in their hydrophilic protein glycan profiles (e.g., melanocytes with melanoma, fibroblasts from different sources).

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