Large-scale experimental studies show unexpected amino acid effects on protein expression and solubility in vivo in E. coli.

(While Gly shows a borderline significant effect on solubility score in the Analysis Dataset used to generate Figure 6 -- Gly does not show a consistent effect on solubility score in the broader collection of datasets analyzed in Figure 4 , while the clearly significant effects in Figure 6 are all generally consistent in those alternative datasets.) Ser and His both have significant negative impacts on solubility score, but not on expression.

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