Novel insights into the distribution and functional aspects of the calcium binding protein secretagogin from studies on rat brain and primary neuronal cell culture.

When synaptogenesis was finished, a strong insulin boost (final concentration: 100 μg/ml) or a gentle – but almost complete – insulin deprivation was performed as described in the Section “Materials and Methods.” Insulin deprivation from normal culture medium over 3 days caused a significant decline in Secretagogin gene expression level to as little as 20% of control levels already on the first day after feeding with insulin-free Neurobasal/B-27 medium (Figure 9 A), whereas a single insulin boost caused a steady highly significant increase in its expression levels after 24 h (Figure 9 B).

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