Heteromeric p97/p97R155C complexes induce dominant negative changes in wild-type and autophagy 9-deficient Dictyostelium strains.

A smaller, however, highly significant increase in plaque size was also seen in AX2 cells expressing p97 R155C -RFP while the expression of wild-type p97-RFP in AX2 wild-type cells had no significant influence ( Fig. 4B ). p97 is Present in Ubiquitin-positive Protein Aggregates in the ATG9 KO but not the ATG9 KO /p97 R155C -RFP Strain In AX2 wild-type cells, immunofluorescence studies employing two polyclonal antibodies that are directed against different regions of the p97 protein revealed a punctate cytoplasmic staining pattern ( Fig. 5A ).

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