When POA explants were grown in the presence of Netrin-1 (400 ng/ml) plus nifedipine (10 μM) GnRH neurite lengths remained at control levels (57.2 μm ±7.1), although there was a slight trend for increased lengths.
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Netrin-1 stimulates developing GnRH neurons to extend neurites to the median eminence in a calcium- dependent manner.
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