PKA and PDE4D3 anchoring to AKAP9 provides distinct regulation of cAMP signals at the centrosome.

Basal cAMP levels are lower at the centrosome than in the bulk cytosol in interphase cells CHO cells expressing the PKA-GFP sensor show a small but highly significant difference in the basal CFP/YFP emission intensity ratio (R) at the centrosome as compared with the bulk cytosol ( Fig. 2 A ), indicating that in resting, nonstimulated interphase cells, the level of cAMP at the centrosome is lower than the mean cAMP level in the cytosol.

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