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Deletional protein engineering based on stable fold.

PLoS One · 2012 · PMC3519881 · PMID 23240034

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an observed trendno p-value reported
An observed trend was that the variants showing almost complete loss of fluorescence lost more amino acid residues and electrostatic interactions around chromophore compared to the variants showing reduced fluorescence.

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Quoted from the open-access full text in Europe PMC under the licence the publisher applied. The sentence is reproduced exactly as published; the emphasis is ours.