Genomic consequences of transitions from cross- to self-fertilization on the efficacy of selection in three independently derived selfing plants.

paradoxa was used as an outgroup to polarize the direction of mutations the trend was similar but marginally insignificant (Nicaragua, deleterious/total = 0.268; Jamaica, deleterious/total = 0.242; Brazil, deleterious/total = 0.225, χ 2 = 5.27, P = 0.07).

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Although GC3s was found to be highly significant, we also present the results of an ANOVA that excluded GC3s because we found that all of our putatively optimal codons have guanine or cytosine in the third position, therefore GC3s was not independent of F op .

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