highly significantP < 0.001
Application of NLC formulations (A1, A2, A3, A4, and A5) for 7 days led to a highly significant increase in average skin hydration from day one to day seven ( P < 0.001), whereas untreated controls remained almost unchanged (30.65 arbitrary units on day one and 31.80 arbitrary units on day seven; P > 0.05) ( Figure 3 ).