The c.1111A allele frequency has shown a tendency to increase from 1990 to 2009 as shown in Figure 5 . Genotype effects on litter size EBVs were highly significant, F-values were 102.84, 102.00 and 98.24 for EBV1, EBV2 and EBV3, respectively.
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A missense mutation in growth differentiation factor 9 (GDF9) is strongly associated with litter size in sheep.
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