Although the values for the individual FRET efficiencies are comparable between samples there does seem to be a slight trend resulting in a reduced measure of ECY in presence of YR and CR/YR, an enhancement of ECR in presence of CY/YR (apparent in Fig. 1A as well) and a reduction of YR when in the presence of CR and CY/CR.
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Simultaneous quantitative live cell imaging of multiple FRET-based biosensors.
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