Enhanced transgene expression in sugarcane by co-expression of virus-encoded RNA silencing suppressors.

Each of these two suppressors resulted in a highly significant improvement in the transient transformation efficiency, as shown by up to a 4.6-fold and 3.6-fold increase in the EYFP expression level at 192 h after bombardment, respectively, when compared to the control (absence of a suppressor) (expression level: 0.70×10 5 ±0.10×10 5 ) ( Figure 2b ).

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