highly significantP < 0.001
Effect of chloroform and petroleum extract of Nigella sativa seeds on infarct volume in MCAO rats TTC dye staining of brains of all extracts pretreated animals showed significant improvement in the infarct volume aspirin, chloroform and petroleum extracts pretreated animals showed a highly significant reduction in infarct volume as compared with MCAO rats ( P < 0.001) [Figures 7 and 8 ].