Saliva sampling in global clinical studies: the impact of low sampling volume on performance of DNA in downstream genotyping experiments.

While saliva sample volume remained the variable with the largest effect (P = 6.8x10 -27 ), DNA turbidity and purity where highly significant at P = 2.1×10 -7 and P = 1.8×10 -4 , respectively.

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Interestingly, a borderline significant association (P = 0.058) was observed between age and saliva volume, suggesting that older subjects may have been more likely to provide a 0.5 mL sample, consistent with reports that xerostomia (dry mouth) prevalence increases with age [ 8 ].

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