A highly significant increase in the AOX signal was detected in both mutants, confirming that leaf respiration relied heavily on the alternative pathway in mtsf1 plants ( Figure 4 B). mtsf1 plants are complex I respiratory mutants To further clarify the respiratory perturbations present in mtsf1 mutants, we analyzed the steady–state levels of the different respiratory complexes in comparison with the wild-type.
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The pentatricopeptide repeat MTSF1 protein stabilizes the nad4 mRNA in Arabidopsis mitochondria.
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