extremely significantP < 0.0001
Statistically, extremely significant difference was observed between the prewash and postwash at RT sperm DNA fragmentation mean percentage values ( t = 5.169, P < 0.0001), similarly statistically significant difference was observed between the prewash and postwash at 37°C sperm DNA fragmentation mean percentage values ( t = 5.090, P < 0.0001).