454 next generation-sequencing outperforms allele-specific PCR, Sanger sequencing, and pyrosequencing for routine KRAS mutation analysis of formalin-fixed, paraffin-embedded samples.

As summarized in Table 3 , we found a highly significant correlation between the degree of tumor cell enrichment evaluated at histology and the abundance of KRAS -mutated DNA for all the techniques, with slightly less significant performance ( P = 0.03) for real-time PCR.

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