Impact of library preparation on downstream analysis and interpretation of RNA-Seq data: comparison between Illumina PolyA and NuGEN Ovation protocol.

PLoS One · 2013 · PMC3747248 · PMID 23977132

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highly significantno p-value reported

Nevertheless, for the highly significant common genes detected in both protocols, a reasonable agreement is achieved and they are validated by RT-PCR.

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an overall trendno p-value reported

There was an overall trend that the detected gene expression was higher from the PolyA protocol than the NuGEN (the fitted lowess curve tilted to x-axis).

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