Profiling the impact of medium formulation on morphology and functionality of primary hepatocytes in vitro.

There was a highly significant decrease in tCYP450 content, from 120 ± 37 pmol/mg protein at day 0 to ≈40 ± 10 pmol/mg protein by day 2 of culture in all media formulations (p < 0.001). tCYP450 content remained quite stable (≈30 ± 3 pmol/mg protein) up to day 4, but had declined significantly, when comparing day 2 values (≈40 ± 10 pmol/mg protein) with day 6 values in WE (17 ± 1 pmol/mg protein; p = 0.047) and ML-15 (25 ± 1 pmol/mg protein; p = 0.021) media formulations.

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