Preamplification procedure for the analysis of ancient DNA samples.

ScientificWorldJournal · 2013 · PMC3800654 · PMID 24187523

Europe PMC DOI

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extremely significantP < 0.0001actually significant

Preamplified samples, as compared to nonpreamplified DNA, showed an extremely significant improvement of the Ct value ( P < 0.0001), ranging from 10.0 to 14.4 for mitochondrial target, suggesting that preamplification step enhances the sensitivity of mitochondrial real-time PCR assay.

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