Slow proton transfer coupled to unfolding explains the puzzling results of single-molecule experiments on BBL, a paradigmatic downhill folding protein.

Such change in stability could be highly significant given that the effect of the ionic denaturant guanidinium chloride (GdmCl) on the pK a of buffers and on the pH reading of glass electrodes implies that the Huang et al. experiments were in fact performed at pH∼8 instead of 7 [35] , compared to the pH 6 of Liu et al.

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