Strengths and weaknesses of recently engineered red fluorescent proteins evaluated in live cells using fluorescence correlation spectroscopy.

There is a possible trend relating the flickering threshold and molecular brightness, and the flickering threshold and measured quantum yield, of the three red FPs from Entacmaea quadricolor (labeled in red in Figure 7 ) (here, the M b used for TagRFP-T is the brightness found in lysate with detergent).

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