HER-2/neu expression was not changed at any of the tested time points by ICC (Additional file 4 : Figure S4, lower panels) but a weak trend towards increased ERBB2 was seen in the first 8 weeks of estrogen deprivation by qRT-PCR (Figure 3 F).
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Clinical instability of breast cancer markers is reflected in long-term in vitro estrogen deprivation studies.
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