High efficiency protocol of DNA extraction from <i>Micromys minutus</i> mandibles from owl pellets: a tool for molecular research of cryptic mammal species.

Results Quantity of DNA The test of the extraction protocols indicated that in the case of Qiagen Kit, on average, 8.15 ng (SE = 0.39, i.e., 815 ng/g of mandible) of DNA was obtained per sample, whereas the Promega protocol provided on average 19.20 ng (SE = 0.455, i.e., 1,920 ng/g of mandible) of DNA, and the difference was highly significant ( t test, t = 18.35, df = 17.65, p < 0.0001; Table 1 ).

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