Dosage and cell line dependent inhibitory effect of bFGF supplement in human pluripotent stem cell culture on inactivated human mesenchymal stem cells.

In addition, bFGF demonstrated a dosage dependent inhibitory effect on freezing/thawing recovery across all cell lines, which was reflected by the statistically significant differences observed between different bFGF culture conditions within each accutased group, as well as an overall trend of decline in the number of colonies recovered from freezing/thawing in response to increased bFGF concentration in culture media within each collagenased group ( Figure 9 and Figure S2 ).

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