highly significantp<0.0001
Cells expressing the control CYC1 3′UTR reporter showed little difference in the population mean fluorescence intensity (MFI) when Ssd1 was absent (0.87-fold change in expression in ssd1Δ compared to SSD1 ), while cells containing the test CTS1 3′UTR construct exhibited a nearly three-fold, highly significant increase (p<0.0001) in MFI in ssd1Δ cells compared to SSD1 cells ( Figure S1A ).