NAD⁺ accumulation as a metabolic off switch for orthodox pollen.

Our results indicate that the NAD-associated redox homeostasis may be significant with regard to pollen desiccation susceptibility.

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Thus, a better understanding of the mechanisms underlying the physiological properties that allow classification of pollen into the categories “orthodox” and “recalcitrant,” based on the status of metabolism and desiccation is fairly significant in the evolution and establishment of reproductive isolation in plants.

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