marginal significanceP = 0.083
Analysis of simple effects revealed a significant difference between morphine and saline groups when the solution concentration was 2.5% [ F (1, 97) = 6.13, P < 0.05], as well as a marginal significance between Morphine-4% and Saline-4% groups [ F (1, 97) = 3.07, P = 0.083].