Meiotic chromosome structures constrain and respond to designation of crossover sites.

Given the fraction of total axis length that was asynapsed in these syp-1 RNAi mnT12 nuclei, the observed restriction of GFP::COSA-1 foci to synapsed segments (where homologs are closely juxtaposed) represents a highly significant (χ 2 test; p = 0.0002) departure from the distribution expected if GFP::COSA-1 foci were equally likely to occur on synapsed segments and asynapsed segments (where homologs are separated), consistent with the interpretation that these GFP::COSA-1 foci correspond to interhomolog recombination events. 6 Extended Data Figure 4.

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