According to the alignment of these DENV-1 and DENV-2 isolates, the N290D, L402F and A473T mutations in the E gene and the R101K, G105R, D340E and L349M mutations in the NS1 gene region of the DENV-1 isolates may be significant, while these AAs in the DENV-2 isolates have not been changed ( Figure 3 ).
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Spatiotemporal characterizations of dengue virus in mainland China: insights into the whole genome from 1978 to 2011.
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A clade supported by a bootstrap value of at least 90% was considered highly significant.