Were the proteins loaded at μM concentrations? For example, in Figure 4A , if one compares the intensities in lanes 13 and 18, there are slight differences which may be significant for a low affinity interaction.
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Were the proteins loaded at μM concentrations? For example, in Figure 4A , if one compares the intensities in lanes 13 and 18, there are slight differences which may be significant for a low affinity interaction.