A decreasing trend was observed for the number of upregulated phosphopeptides, which decreased from 53.7% in the 1 h group to 40.0% in the 6 h group.
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Quantitative phosphoproteomics analysis of nitric oxide-responsive phosphoproteins in cotton leaf.
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We also included the cutoff for the log 2 fold change values, in which the phosphorylation changes were considered highly significant if the log 2 value ≥ 0.58 or ≤−0.58 (increasing or decreasing 1.5 fold in phosphorylation activity).